Background: Striae distensae, or stretch marks, are common dermal lesions resulting from skin stretching and are often associated with aesthetic concerns and psychological distress. Fractional CO₂ laser therapy has been mostly used as a promising treatment for promoting collagen remodeling and improving skin texture with minimal time. Method: A prospective observational study was done in the Department of Dermatology at Sri Siddhartha Medical College, Tumkur, to evaluate the efficacy of fractional CO₂ laser in treating striae distensae. Patients with >18 years and clinically diagnosed with striae alba were enrolled for the study. Each patient underwent up to three sessions of fractional CO₂ laser at four-week intervals. Clinical improvement was assessed using a global improvement scale, changes in striae surface area, and a Visual Analog Scale (VAS) for patient satisfaction. Adverse effects were also recorded. Results: Most patients showed significant clinical improvement post-treatment, with reductions in striae surface area and high patient satisfaction. The majority reported moderate to good improvement, and only a few experienced minimal changes. Adverse effects were generally mild and self-limiting. Conclusion: Fractional CO₂ laser therapy is an effective, safe, and well-tolerated modality for the treatment of striae distensae, offering substantial clinical and cosmetic benefits.